anti p stat3 (Cell Signaling Technology Inc)

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Cell Signaling Technology Inc anti p stat3
Anti P Stat3, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 96/100, based on 1298 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/anti+p+stat3/Stat3+Rabbit+mAb/pm42817007-337-38-40
Average 96 stars, based on 1298 article reviews

anti p stat3 - by Bioz Stars, 2026-10

96/100 stars

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Incubation:

Article Title: Ruxolitinib reverses alopecia areata via a triple mechanism: JAK-STAT inhibition, localized oxidative stress attenuation and selective apoptosis modulation
Article Snippet: .. Sections were blocked with 10% normal goat serum for 30 min at room temperature and then incubated overnight at 4 ◦C with the following primary antibodies: anti-CD8 (1:200 dilution; Cell Signaling Technology, 98941s), anti-p-STAT1 (1: 250; Bioss Antibodies, 52209R), and anti-p-Stat3 (1:50 dilution; Cell Signaling Technology, 9145s). .. After washing, sections were incubated with a biotinylated goat anti-rabbit IgG secondary antibody (1:200 dilution; Vector Laboratories, BA-1000) for 1 h at room temperature, followed by incubation with an ABC-HRP complex (Vector Laboratories, PK-4000) for 30 min. Color development was performed using a DAB peroxidase substrate kit (Vector Laboratories, SK-4100), and the reaction was monitored under a microscope.

Article Title: Regorafenib and Nifuroxazide exert enhanced suppression of hepatocellular carcinoma by inhibiting STAT3 and immune remodeling
Article Snippet: A total of 10 μg protein per lane was separated via SDS-PAGE using 10% Tris-Gly SDS-PAGE precast gels (Beyotime Biotechnology; cat. no. P0052A), followed by transfer to PVDF membranes (MilliporeSigma) at 220 mA. .. Membranes were blocked with 5% skimmed milk in 0.05% Tween-20 at room temperature for 1 h, then incubated overnight at 4°C with one of the following primary antibodies: anti-PD-L1 (1:1,000; Shanghai Abways Biotechnology Co., Ltd.; cat. no. CY5980); anti-VEGF (1:2,000, Affinity Biosciences, Ltd.; cat. no. AF5131); anti-p-STAT3 (1:1,000, Cell Signaling Technology, Inc.; cat. no. 9145); anti-STAT3 (1:1,000, Cell Signaling Technology, Inc.; cat. no. 9139); anti-Bcl-2 (1:2,000, Shanghai Abways Biotechnology Co., Ltd.; cat. no. CY6717); anti-Bax (1:2,000, Shanghai Abways Biotechnology Co., Ltd.; cat. no. CY5059); anti-MMP2 (1:1,000, Cell Signaling Technology, Inc.; cat. no. 40994); anti-cyclin D1 (1:2,000, Santa Cruz Biotechnology, Inc.; cat. no. sc-8396); anti-cleaved-caspase 3 (1:2,000, Cell Signaling Technology; cat. no. 9664); anti-caspase 3 (1:2,000, Cell Signaling Technology; cat. no. 9662); phospho-JAK2 (1:1,000; UpingBio technology Co, Ltd.; cat. no. YP-Ab-14423); JAK2 (1:1,000; UpingBio technology Co, Ltd.; cat. no. YP-Ab-14800); SHP-1 (1:1,000; UpingBio technology Co, Ltd.; cat. no. YP-mAb-14236); anti-Tubulin (1:4,000, MilliporeSigma; cat. no. T9026); and anti-GAPDH (1:4,000, MilliporeSigma; cat. no. G8795) according to the manufacturer's protocol. .. Following washing, membranes were incubated with a horseradish peroxidase (HRP)-conjugated secondary antibody (1:5,000; OriGene Technologies, Inc.; cat. nos.

Article Title: Endogenous Ceramide 24:1 Constrains Th17-Driven Neutrophilic Inflammation by Antagonizing EP2 Signaling.
Article Snippet: Advanced Science, 2026 om m ons L icense nloaded from https://advanced.onlinelibrary.w iley.com /doi/10.1002/advs.202520695 by IN A SP - N E PA L , W iley O nline L ibrary on [15/03/2026]. .. See the T erm s and C onditions (https://onlinelibrary.w iley.com /term s-and-conditions) on W iley O nline L ibrary for rules of use; O A articles are governed by the applicable C reative C For WB analysis, the protein lysates were separated via SDS ‒PAGE, transferred to PVDF membranes, and incubated overnight at 4◦C with the following primary antibodies: antiSmpd1 (1:1000, ABclonal, #39147-1), anti-Cers2 (1:1000, Abcam, #ab31545), anti-Sptlc2 (1:1000, Abcam, #ab229330), anti-Ror γt (1:1000, Proteintech, #29910-1-AP), anti-Jak2 (1:1000, CST, #3230), anti-p-Jak2 (1:1000, CST, #8082S), anti-Stat3 (1:1000, CST, #4904T), anti-p-Stat3 (1:1000, CST, #9145T), anti-EP2 (1:1000, Abcam, #ab167171), anti-EP4 (1:1000, Proteintech, #24895-1-AP), anti-CXCR4 (1:1000, Abcam, #ab181020), anti-CREB (1:1000, CST, #9197), anti-p-CREB (1:1000, CST, #9198), and anti- βActin (1:1000, Proteintech, #20536-1-AP). ..

other:

Article Title: Rhomboid protease RHBDL2 is a calcium-activated suppressor of EGFR signalling in keratinocytes
Article Snippet: Anti-EGFR Y992 (Cat. No. 2235) and Y1068P (3777), anti-P-Stat3 (9145), anti-Stat3 (12640), anti-P-Akt (9271), anti-PLCγ-1 (5690), anti-P-PLCγ-1 (14008), anti-tubulin (3873), anti-FLAG-tag (2368), anti-HA-tag (3724) and anti-Myc-tag (2278) were from Cell Signalling Technology (USA).

Article Title: KAT8 drives M2 macrophage polarization to exacerbate allergic airway inflammation
Article Snippet: Anti-p-STAT3 , Cell Signaling Technology , Cat#9145; RRID: AB_2491009.

Western Blot:

Nucleic Acid Electrophoresis:

Article Title: Parameter-screened LED therapy targets STAT3/IL-6 axis to attenuate tendinopathy via dual modulation of inflammation and ECM remodeling.
Article Snippet: Cells were lysed in RIPA buffer (R0020; Solarbio, Beijing, China) with protease/phosphatase inhibitors (P1261; Solarbio). .. Proteins were separated by 10% sodium dodecyl sulfatepolyacrylamide gel electrophoresis (SDS-PAGE), transferred to polyvinylidene difluoride (PVDF) membranes (Millipore, Merck KgaA, Darmstadt, Germany), and probed overnight at 4°C with primary antibodies: anti-COL1 (ab270993; Abcam, Cambridge, UK, 1:1000), antiCOL3 (ab184993; Abcam, 1:1000), anti-lubricin (bs-11175R, Bioss, Beijing, China, 1:1000), anti-p-STAT3 (9145; Cell Signaling Technology, Danvers, MA, USA, 1:1000), anti-STAT3 (9139; Cell Signaling Technology, 1:1000), and anti-β-actin (AF5003; Beyotime, Shanghai, China, 1:5000). .. Horseradish peroxidase-conjugated secondary antibodies (A0208, A0216; Beyotime, 1:5000) were applied, followed by enhanced chemiluminescence detection (PE0010; Solarbio).

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